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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Moesin Polyclonal antibody (26053-1-AP)

    WB analysis of Jurkat using 26053-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Moesin Polyclonal antibody (26053-1-AP)

    WB analysis using 26053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Moesin Polyclonal antibody (26053-1-AP)

    WB analysis of HeLa using 26053-1-AP

    WB result of Moesin antibody (26053-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using Moesin Polyclonal antibody (26053-1-AP)

    WB analysis of HeLa using 26053-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Moesin Polyclonal antibody (26053-1-AP)

    IHC staining of mouse kidney using 26053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Moesin Polyclonal antibody (26053-1-AP)

    IHC staining of human colon using 26053-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26053-1-AP (Moesin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Moesin Polyclonal antibody (26053-1-AP)

    IHC staining of mouse kidney using 26053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Moesin Polyclonal antibody (26053-1-AP)

    IF Staining of HepG2 using 26053-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (26053-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

Moesin Polyclonal antibody

Moesin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26053-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MSN, Membrane-organizing extension spike protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, Neuro-2a cells, C6 cells, mouse heart tissue, mouse lung tissue, rat heart tissue
Positive IHC detected inhuman colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26053-1-AP targets Moesin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Moesin fusion protein Ag23531 相同性解析による交差性が予測される生物種
Full Name moesin
Calculated molecular weight 577 aa, 68 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC017293
Gene Symbol Moesin
Gene ID (NCBI) 4478
RRIDAB_2880353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26038
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Protocols

Product Specific Protocols
WB protocol for Moesin antibody 26053-1-APDownload protocol
IHC protocol for Moesin antibody 26053-1-APDownload protocol
IF protocol for Moesin antibody 26053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols