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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP8 Polyclonal antibody (29398-1-AP)

    WB analysis using 29398-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29398-1-AP (MT-ATP8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 143B.TK.P0 cells using ATP8 Polyclonal antibody (29398-1-AP)

    WB analysis of 143B.TK.P0 using 29398-1-AP

    WB result of ATP8 antibody (ATP8; 1:1000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

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  • Featured Product
  • KD/KO Validated

ATP8 Polyclonal antibody

ATP8 Polyclonal Antibody for WB, ELISA
Cat No. 29398-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MT-ATP8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, 143B cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29398-1-AP targets ATP8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP8 fusion protein Ag30024 相同性解析による交差性が予測される生物種
Full Name ATP synthase 8; ATPase subunit 8
Calculated molecular weight 8 kDa
Observed molecular weight8-10 kDa
Gene Symbol ATP8
Gene ID (NCBI) 4509
RRIDAB_2918293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP03928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP8 antibody 29398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols