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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human testis using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human testis using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human testis using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12179-1-AP (MT3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human kidney using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human kidney using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MT3 Polyclonal antibody (12179-1-AP)

    IHC staining of human brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

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MT3 Polyclonal antibody

MT3 Polyclonal Antibody for IHC, ELISA
Cat No. 12179-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, ELISA

Synonyms

GIF, GIFB, GRIF, Growth inhibitory factor, metallothionein 3, Metallothionein III, MT 3, MT III, MT3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IHC detected inmouse brain tissue, human brain tissue, human testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12179-1-AP targets MT3 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MT3 fusion protein Ag2823 相同性解析による交差性が予測される生物種
Full Name metallothionein 3
Calculated molecular weight 68 aa, 7 kDa
GenBank accession numberBC013081
Gene Symbol MT3
Gene ID (NCBI) 4504
RRIDAB_2146645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25713
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MT3(Metallothionein-3) is also names as GIF(Growth inhibitory factor),GIFB inhibits N-glycosylation of IgE-binding factors and that unglycosylated IgE-binding factors selectively suppressed IgE synthesis.The major cell source of GIF is antigen-specific suppressor T(Ts)cell(PMID:10967025).The molecular size of GIF from T cells is 15 kDa, while GIF from macrophages consisted of 40-and 15-kDa molecules(PMID:2417235).

Protocols

Product Specific Protocols
IHC protocol for MT3 antibody 12179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pig

Int J Mol Sci

Integrating Transcriptomics and Proteomics to Characterize the Intestinal Responses to Cadmium Exposure Using a Piglet Model

Authors - Yikun Li
View Article