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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTA1 Polyclonal antibody

MTA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30545-1-AP

Synonyms

MTA 1, metastasis associated 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MTA1 Polyclonal antibody (30545-1-AP)

    WB analysis using 30545-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MTA1 Polyclonal antibody (30545-1-AP)

    WB analysis using 30545-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human prostate cancer using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human prostate cancer using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human skin cancer using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of human skin cancer using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of rat cerebellum using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using MTA1 Polyclonal antibody (30545-1-AP)

    IHC staining of rat cerebellum using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MTA1 Polyclonal antibody (30545-1-AP)

    IF Staining of MCF-7 using 30545-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MTA1 antibody (30545-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, HEK-293T cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human skin cancer tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30545-1-AP targets MTA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTA1 fusion protein Ag30994 相同性解析による交差性が予測される生物種
Full Name metastasis associated 1
Calculated molecular weight 81 kDa
Observed molecular weight70-80 kDa
GenBank accession numberNM_004689
Gene symbol MTA1
Gene ID (NCBI) 9112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Metastasis-associated protein 1 (MTA1) is a member of the MTA family which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins(PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancer.

Protocols

Product Specific Protocols
WB protocol for MTA1 antibody 30545-1-APDownload protocol
IHC protocol for MTA1 antibody 30545-1-APDownload protocol
IF protocol for MTA1 antibody 30545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Authors - Sihang Zhou
View Article