Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 30545-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 30545-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 30545-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 30545-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 30545-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 30545-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 30545-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 30545-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 30545-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MTA1 antibody (30545-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

MTA1 Polyclonal antibody

MTA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30545-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MTA 1, metastasis associated 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, NIH/3T3 cells, HEK-293T cells, MCF-7 cells
Positive IHC detected in	human prostate cancer tissue, human skin cancer tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

30545-1-AP targets MTA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MTA1 fusion protein Ag30994 相同性解析による交差性が予測される生物種
Full Name	metastasis associated 1
Calculated molecular weight	81 kDa
Observed molecular weight	70-80 kDa
GenBank accession number	NM_004689
Gene Symbol	MTA1
Gene ID (NCBI)	9112
RRID	AB_3086358
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13330
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Metastasis-associated protein 1 (MTA1) is a member of the MTA family which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins(PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancer.

Protocols

Product Specific Protocols
WB protocol for MTA1 antibody 30545-1-AP	Download protocol
IHC protocol for MTA1 antibody 30545-1-AP	Download protocol
IF protocol for MTA1 antibody 30545-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	bioRxiv Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations Authors - Sihang Zhou View Article

Target

Host

Species Reactivity