Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse thymus tissue, HEK-293 cells |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | rat lung tissue, human heart tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
17554-1-AP targets MTA2 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MTA2 fusion protein Ag11713 相同性解析による交差性が予測される生物種 |
| Full Name | metastasis associated 1 family, member 2 |
| Calculated molecular weight | 668 aa, 75 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC053650 |
| Gene Symbol | MTA2 |
| Gene ID (NCBI) | 9219 |
| RRID | AB_10638601 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94776 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTA2 antibody 17554-1-AP | Download protocol |
| IHC protocol for MTA2 antibody 17554-1-AP | Download protocol |
| IP protocol for MTA2 antibody 17554-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level. | ||
Cell Mol Gastroenterol Hepatol Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.
| ||
Cancer Sci MTA2 promotes the metastasis of esophageal squamous cell carcinoma via EIF4E-Twist feedback loop. | ||
Nucleic Acids Res CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development |