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MTA2 Polyclonal antibody

MTA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 17554-1-AP

Synonyms

Metastasis associated 1 like 1, MTA1 L1 protein, MTA1L1, MTA2, PID


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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse thymus tissue, HEK-293 cells
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17554-1-AP targets MTA2 in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTA2 fusion protein Ag11713 相同性解析による交差性が予測される生物種
Full Name metastasis associated 1 family, member 2
Calculated molecular weight 668 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC053650
Gene symbol MTA2
Gene ID (NCBI) 9219
RRIDAB_10638601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Protocols

Product Specific Protocols
WB protocol for MTA2 antibody 17554-1-APDownload protocol
IHC protocol for MTA2 antibody 17554-1-APDownload protocol
IP protocol for MTA2 antibody 17554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
humanWB

EMBO Rep

miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level.

Authors - Dapeng Wang
humanWB

Cell Mol Gastroenterol Hepatol

Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.

Authors - Gui-Qi Zhu
  • KD Validated
CoIP,ChIP

Cancer Sci

MTA2 promotes the metastasis of esophageal squamous cell carcinoma via EIF4E-Twist feedback loop.

Authors - Su-Li Dai
humanWB

Nucleic Acids Res

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development

Authors - Xue Chen