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- KD/KO Validated
MTA3 Polyclonal antibody
MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14682-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, mouse thymus tissue, Jurkat cells, MCF-7 cells, Raji cells |
Positive IP detected in | Raji cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U-251 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
IHC | See 9 publications below |
IP | See 1 publications below |
Product Information
14682-1-AP targets MTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MTA3 fusion protein Ag6400 相同性解析による交差性が予測される生物種 |
Full Name | metastasis associated 1 family, member 3 |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 62 kDa, 68 kDa |
GenBank accession number | BC053631 |
Gene symbol | MTA3 |
Gene ID (NCBI) | 57504 |
RRID | AB_2298003 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
Protocols
Product Specific Protocols | |
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WB protocol for MTA3 antibody 14682-1-AP | Download protocol |
IHC protocol for MTA3 antibody 14682-1-AP | Download protocol |
IF protocol for MTA3 antibody 14682-1-AP | Download protocol |
IP protocol for MTA3 antibody 14682-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Stem Cell The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development. | ||
Mol Cell The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. | ||
Oncotarget Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis. | ||
PLoS One Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.
| ||
J Gastroenterol Hepatol Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma. | ||
Tumour Biol MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway. |