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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTAP Recombinant antibody (83005-5-RR)

    IF Staining of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTAP antibody (83005-5-RR, Clone: 240030E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83005-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MTAP Recombinant antibody (83005-5-RR)

    FC experiment of HeLa using 83005-5-RR (same clone as 83005-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTAP Recombinant antibody (83005-5-RR, Clone:240030E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83005-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83005-5-RR

    Affinity and Kinetic Characterization of 83005-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-5-RR against Human MTAP were performed. The affinity constant is 1.54 nM.

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MTAP Recombinant antibody, PBS Only

MTAP Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 83005-5-PBS
Clone No.240030E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

MTA phosphorylase, MSAP, EC:2.4.2.28, c86fus, 5'-methylthioadenosine phosphorylase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83005-5-PBS targets MTAP in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTAP fusion protein Ag2051 相同性解析による交差性が予測される生物種
Full Name methylthioadenosine phosphorylase
Calculated molecular weight 283 aa, 31 kDa
GenBank accession numberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13126
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.