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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTCL1 Polyclonal antibody (25780-1-AP)

    WB analysis using 25780-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25780-1-AP (MTCL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using MTCL1 Polyclonal antibody (25780-1-AP)

    IP experiment of A549 using 25780-1-AP

    IP result of anti-KIAA0802 (IP:25780-1-AP, 4ug; Detection:25780-1-AP 1:400) with A549 cells lysate 640 ug.

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MTCL1 Polyclonal antibody

MTCL1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25780-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IP, ELISA

Synonyms

SOGA2, KIAA0802, CCDC165

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MDCK cells
Positive IP detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25780-1-AP targets MTCL1 in WB, IP, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTCL1 fusion protein Ag22718 相同性解析による交差性が予測される生物種
Full Name KIAA0802
Calculated molecular weight 1905 aa, 209 kDa
Observed molecular weight250 kDa
GenBank accession numberBC040542
Gene Symbol MTCL1
Gene ID (NCBI) 23255
RRIDAB_3669499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4B5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microtubule cross‐linking factor 1 (MTCL1) is a MT‐regulating protein that plays essential roles in accumulating the non-centrosomal MTs into the apicobasal MT bundles. MTCL1, identified as a binding protein of the cell polarity‐regulating kinase, PAR‐1b/MARK2, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Western blot analysis detected MTCL1 at an apparent molecular mass of 250 kDa as indicated in the literature (PMID: 23902687, 28283581).

Protocols

Product Specific Protocols
WB protocol for MTCL1 antibody 25780-1-APDownload protocol
IP protocol for MTCL1 antibody 25780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols