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Validation Data Gallery

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  • Western Blot (WB) analysis of 143B.TK.P0 cells using MTCO2 Monoclonal antibody (68527-1-Ig)

    WB analysis of 143B.TK.P0 using 68527-1-Ig (same clone as 68527-1-PBS)

    WB result of MTCO2 antibody (MTCO2; 1:1000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line. This data was developed using the same antibody clone with 68527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTCO2 Monoclonal antibody (68527-1-Ig)

    WB analysis using 68527-1-Ig (same clone as 68527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68527-1-Ig (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68527-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MTCO2 Monoclonal antibody, PBS Only

MTCO2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68527-1-PBS
Clone No.10G14F4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

COX2, MT CO2, COXII, COX 2, COII

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68527-1-PBS targets MTCO2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase II
Calculated molecular weight 26 kDa
Observed molecular weight 23-26 kDa
GenBank accession numberYP_003024029
Gene Symbol MTCO2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00403
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).