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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTCO2 Recombinant antibody, PBS Only

MTCO2 Recombinant Antibody for WB, IP, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230448C9

Cat no : 83132-7-PBS

Synonyms

COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTCO2 Recombinant antibody (83132-7-RR)

    WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MTCO2 Recombinant antibody (83132-7-RR)

    IP experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

    IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTCO2 Recombinant antibody (83132-7-RR)

    IF Staining of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83132-7-PBS targets MTCO2 in WB, IP, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase II
Calculated molecular weight 26 kDa
Observed molecular weight 23-26 kDa
GenBank accession numberYP_003024029
Gene symbol COX2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).