Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, C2C12 cells, mouse liver tissue, rat kidney tissue, rat liver tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 20 publications below |
| IF | See 2 publications below |
Product Information
55082-1-AP targets MTCO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome c oxidase III |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 32-35 kDa |
| GenBank accession number | YP_003024032 |
| Gene Symbol | MTCO3 |
| Gene ID (NCBI) | 4514 |
| RRID | AB_2881265 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00414 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTCO3 antibody 55082-1-AP | Download protocol |
| IHC protocol for MTCO3 antibody 55082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Diabetes Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis. | ||
Int J Mol Sci Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice. | ||
J Med Genet COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency. | ||
Sci China Life Sci Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival. | ||