Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 13860-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13860-1-AP
WB result of AEG-1/MTDH-Specific antibody (13860-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AEG-1/MTDH-Specific transfected HeLa cells.
WB analysis of A549 cells using 13860-1-AP
WB result of AEG-1/MTDH antibody (13860-1-AP, 1:2000) with si-Control and si-MTDH transfected A549 cells.
WB analysis of A375 using 13860-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 13860-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 13860-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13860-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 13860-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 13860-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13860-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13860-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 13860-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 13860-1-AP
IP result of anti-AEG-1/MTDH-Specific (IP:13860-1-AP, 3ug; Detection:13860-1-AP 1:1000) with MCF-7 cells lysate 3250ug.
IHC staining of human breast cancer using 13860-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13860-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13860-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13860-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 13860-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 13860-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13860-1-AP (AEG-1/MTDH-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 13860-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AEG-1/MTDH-Specific antibody (13860-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

AEG-1/MTDH Polyclonal antibody

AEG-1/MTDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13860-1-AP

Publications(54)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

AEG-1/MTDH-Specific, AEG-1, MTDH, Astrocyte elevated gene-1 protein, AEG1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (54)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, A375 cells, HeLa cells, DU 145 cells, HL-60 cells, A549 cells, mouse spleen tissue, PC-3 cells, HepG2 cells, RAW 264.7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue, mouse brain tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 43 publications below
IHC	See 24 publications below
IF	See 5 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

13860-1-AP targets AEG-1/MTDH in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AEG-1/MTDH fusion protein Ag4840 相同性解析による交差性が予測される生物種
Full Name	metadherin
Calculated molecular weight	582 aa, 64 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC045642
Gene Symbol	AEG-1/MTDH
Gene ID (NCBI)	92140
RRID	AB_2266734
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86UE4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for AEG-1/MTDH antibody 13860-1-AP	Download protocol
IHC protocol for AEG-1/MTDH antibody 13860-1-AP	Download protocol
IF protocol for AEG-1/MTDH antibody 13860-1-AP	Download protocol
IP protocol for AEG-1/MTDH antibody 13860-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,rat	WB	Environ Int Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels Authors - Qiuju Liang View Article
human	IF, WB, IP, IHC	J Crohns Colitis MTDH promotes intestinal inflammation by positively regulating TLR signaling. Authors - Lijuan Wang View Article
human	WB	Oncogene MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo. Authors - He X-X XX View Article
		Mol Ther Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells. Authors - Hao Teng View Article
human	WB	Mol Ther Nucleic Acids PMID: 28624193 Authors - Yin-Ping Fan View Article
human	WB,IHC	Oncotarget MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1. Authors - Jing-Jun Yan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 13860-1-AP

WB analysis of HeLa using 13860-1-AP

WB analysis of A549 cells using 13860-1-AP

WB analysis of A375 using 13860-1-AP

WB analysis of RAW 264.7 using 13860-1-AP

WB analysis of PC-3 using 13860-1-AP

WB analysis of HepG2 using 13860-1-AP

WB analysis using 13860-1-AP

WB analysis of mouse spleen using 13860-1-AP

WB analysis of HeLa using 13860-1-AP

WB analysis of A549 using 13860-1-AP

WB analysis of DU 145 using 13860-1-AP

IP experiment of MCF-7 using 13860-1-AP

IHC staining of human breast cancer using 13860-1-AP

IHC staining of human breast cancer using 13860-1-AP

IHC staining of human liver cancer using 13860-1-AP

IHC staining of mouse brain using 13860-1-AP

IHC staining of mouse brain using 13860-1-AP

IHC staining of human liver cancer using 13860-1-AP

IF Staining of MCF-7 using 13860-1-AP

AEG-1/MTDH Polyclonal antibody

AEG-1/MTDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels

MTDH promotes intestinal inflammation by positively regulating TLR signaling.

MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.

Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells.

PMID: 28624193

MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.