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- KD/KO Validated
MTF2 Polyclonal antibody
MTF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 16208-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, mouse ES cells and 293 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | mouse ES cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 21 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| ChIP | See 5 publications below |
Product Information
16208-1-AP targets MTF2 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MTF2 fusion protein Ag9182 相同性解析による交差性が予測される生物種 |
| Full Name | metal response element binding transcription factor 2 |
| Calculated molecular weight | 536 aa, 61 kDa |
| Observed molecular weight | 67 kDa, 60 kDa, 60-61 kDa |
| GenBank accession number | BC010013 |
| Gene symbol | MTF2 |
| Gene ID (NCBI) | 22823 |
| RRID | AB_2147370 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTF2 antibody 16208-1-AP | Download protocol |
| IHC protocol for MTF2 antibody 16208-1-AP | Download protocol |
| IP protocol for MTF2 antibody 16208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding. | ||
Nat Genet RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells. | ||
Nat Cell Biol Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes | ||
Mol Cell Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells. |
