MTF2 Monoclonal antibody
MTF2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Rat, Rabbit, Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2E5D5
Cat no : 68713-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Rabbit testis tissue, Mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68713-1-Ig targets MTF2 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit, Mouse samples.
| Tested Reactivity | Human, Rat, Rabbit, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MTF2 fusion protein Ag9384 相同性解析による交差性が予測される生物種 |
| Full Name | metal response element binding transcription factor 2 |
| Calculated molecular weight | 536 aa, 61 kDa |
| Observed molecular weight | 55 kDa, 60 kDa, 75 kDa |
| GenBank accession number | BC010013 |
| Gene symbol | MTF2 |
| Gene ID (NCBI) | 22823 |
| RRID | AB_3670406 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTF2 antibody 68713-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
