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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTF2 Monoclonal antibody (68713-1-Ig)

    WB analysis using 68713-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68713-1-Ig (MTF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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MTF2 Monoclonal antibody

MTF2 Monoclonal Antibody for WB, ELISA
Cat No. 68713-1-Ig
Clone No.2E5D5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Rabbit, Mouse

Applications

WB, ELISA

Synonyms

dJ976O13.2, hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Rabbit testis tissue, Mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68713-1-Ig targets MTF2 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit, Mouse samples.

Tested Reactivity Human, Rat, Rabbit, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MTF2 fusion protein Ag9384 相同性解析による交差性が予測される生物種
Full Name metal response element binding transcription factor 2
Calculated molecular weight 536 aa, 61 kDa
Observed molecular weight55 kDa, 60 kDa, 75 kDa
GenBank accession numberBC010013
Gene Symbol MTF2
Gene ID (NCBI) 22823
RRIDAB_3670406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y483
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

Protocols

Product Specific Protocols
WB protocol for MTF2 antibody 68713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols