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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTF2 Recombinant antibody

MTF2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230138D10

Cat no : 82944-1-RR

Synonyms

MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96, hPCl2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTF2 Recombinant antibody (82944-1-RR)

    WB analysis using 82944-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82944-1-RR (MTF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTF2 Recombinant antibody (82944-1-RR)

    IF Staining of HeLa using 82944-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTF2 antibody (82944-1-RR, Clone: 230138D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using MTF2 Recombinant antibody (82944-1-RR)

    FC experiment of HEK-293 using 82944-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug MTF2 Recombinant antibody (82944-1-RR, Clone:230138D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82944-1-RR

    Affinity and Kinetic Characterization of 82944-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82944-1-RR against Human MTF2 were performed. The affinity constant is 0.398 nM.

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Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82944-1-RR targets MTF2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTF2 fusion protein Ag9384 相同性解析による交差性が予測される生物種
Full Name metal response element binding transcription factor 2
Calculated molecular weight 536 aa, 61 kDa
Observed molecular weight55 kDa, 60 kDa, 75 kDa
GenBank accession numberBC010013
Gene symbol MTF2
Gene ID (NCBI) 22823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

Protocols

Product Specific Protocols
WB protocol for MTF2 antibody 82944-1-RRDownload protocol
IF protocol for MTF2 antibody 82944-1-RRDownload protocol
FC protocol for MTF2 antibody 82944-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols