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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTF2 Recombinant antibody

MTF2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230138D10

Cat no : 82944-1-RR

Synonyms

MTF 2, Metal-response element-binding transcription factor 2, Metal-response element DNA-binding protein M96, M96, hPCl2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTF2 Recombinant antibody (82944-1-RR)

    WB analysis using 82944-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82944-1-RR (MTF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTF2 Recombinant antibody (82944-1-RR)

    IF Staining of HeLa using 82944-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTF2 antibody (82944-1-RR, Clone: 230138D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using MTF2 Recombinant antibody (82944-1-RR)

    FC experiment of HEK-293 using 82944-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug MTF2 Recombinant antibody (82944-1-RR, Clone:230138D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82944-1-RR

    Affinity and Kinetic Characterization of 82944-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82944-1-RR against Human MTF2 were performed. The affinity constant is 0.398 nM.

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Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82944-1-RR targets MTF2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTF2 fusion protein Ag9384 相同性解析による交差性が予測される生物種
Full Name metal response element binding transcription factor 2
Calculated molecular weight 536 aa, 61 kDa
Observed molecular weight55 kDa, 60 kDa, 75 kDa
GenBank accession numberBC010013
Gene symbol MTF2
Gene ID (NCBI) 22823
RRIDAB_3670687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

Protocols

Product Specific Protocols
WB protocol for MTF2 antibody 82944-1-RRDownload protocol
IF protocol for MTF2 antibody 82944-1-RRDownload protocol
FC protocol for MTF2 antibody 82944-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols