- Featured Product
- KD/KO Validated
MTHFD1 Polyclonal antibody
MTHFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10794-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HuH-7 cells, human brain tissue, K-562 cells, mouse kidney tissue, MCF-7 cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 12 publications below |
IHC | See 1 publications below |
Product Information
10794-1-AP targets MTHFD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MTHFD1 fusion protein Ag1225 相同性解析による交差性が予測される生物種 |
Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase |
Calculated molecular weight | 101 kDa |
Observed molecular weight | 101 kDa |
GenBank accession number | BC009806 |
Gene symbol | MTHFD1 |
Gene ID (NCBI) | 4522 |
RRID | AB_2147391 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MTHFD1 antibody 10794-1-AP | Download protocol |
IHC protocol for MTHFD1 antibody 10794-1-AP | Download protocol |
IF protocol for MTHFD1 antibody 10794-1-AP | Download protocol |
IP protocol for MTHFD1 antibody 10794-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. | ||
Redox Biol Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis. | ||
Proc Natl Acad Sci U S A Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
| ||
Dev Cell Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor. | ||
Cell Death Dis MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hua (Verified Customer) (08-26-2019) | Works well.
|