Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10794-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10794-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10794-1-AP
WB result of MTHFD1 antibody (10794-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTHFD1 transfected HEK-293 cells.
WB analysis using 10794-1-AP
HuH-7 cells were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10794-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 10794-1-AP
IP result of anti-MTHFD1 (IP:10794-1-AP, 5ug; Detection:10794-1-AP 1:600) with mouse kidney tissue lysate 4000ug.
IHC staining of human liver cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10794-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 10794-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (10794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

MTHFD1 Polyclonal antibody

MTHFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10794-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C-1-tetrahydrofolate synthase, cytoplasmic, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C1-THF synthase, Epididymis secretory sperm binding protein, MTHFC

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HuH-7 cells, human brain tissue, K-562 cells, mouse kidney tissue, MCF-7 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 18 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

10794-1-AP targets MTHFD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1225 Product name: Recombinant human MTHFD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 536-935 aa of BC009806 Sequence: TNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF 相同性解析による交差性が予測される生物種
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Calculated molecular weight	101 kDa
Observed molecular weight	101 kDa
GenBank accession number	BC009806
Gene Symbol	MTHFD1
Gene ID (NCBI)	4522
RRID	AB_2147391
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11586
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Protocols

Product Specific Protocols
IF protocol for MTHFD1 antibody 10794-1-AP	Download protocol
IHC protocol for MTHFD1 antibody 10794-1-AP	Download protocol
IP protocol for MTHFD1 antibody 10794-1-AP	Download protocol
WB protocol for MTHFD1 antibody 10794-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Oxidative stress inhibits distant metastasis by human melanoma cells. Authors - Elena Piskounova KD Validated View Article
human	WB	Nat Commun Mitochondrial one-carbon metabolism is required for TGF-β-induced glycine synthesis and fibrotic responses. Authors - Angelo Y. Meliton KD Validated View Article
mouse,human	WB,IHC	Nat Commun Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging. Authors - Dan Wu View Article
human	WB,IF	Nat Commun Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues. Authors - S. Kourtis View Article
human	WB	Redox Biol Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis. Authors - Chang Shao View Article
		Mol Cell Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle. Authors - Mushtaq A. Nengroo View Article

Validation Data Gallery

WB analysis using 10794-1-AP

WB analysis using 10794-1-AP

WB analysis of HEK-293 using 10794-1-AP

WB analysis using 10794-1-AP

WB analysis of human brain using 10794-1-AP

IP experiment of mouse kidney using 10794-1-AP

IHC staining of human liver cancer using 10794-1-AP

IHC staining of human liver cancer using 10794-1-AP

IHC staining of mouse liver using 10794-1-AP

IHC staining of mouse liver using 10794-1-AP

IHC staining of human colon cancer using 10794-1-AP

IHC staining of human liver cancer using 10794-1-AP

IHC staining of human liver cancer using 10794-1-AP

IHC staining of human colon cancer using 10794-1-AP

IHC staining of human colon cancer using 10794-1-AP

IF Staining of HEK-293 using 10794-1-AP

MTHFD1 Polyclonal antibody

MTHFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Oxidative stress inhibits distant metastasis by human melanoma cells.

Mitochondrial one-carbon metabolism is required for TGF-β-induced glycine synthesis and fibrotic responses.

Elevating cytosolic NADPH metabolism in endothelial cells ameliorates vascular aging.

Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.

Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.

Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle.

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