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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFD1 Recombinant antibody (84884-5-RR)

    WB analysis using 84884-5-RR (same clone as 84884-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84884-5-RR (MTHFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84884-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84884-5-RR

    Affinity and Kinetic Characterization of 84884-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84884-5-RR against Human MTHFD1 were performed. The affinity constant is below 1 pM.

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MTHFD1 Recombinant antibody, PBS Only

MTHFD1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84884-5-PBS
Clone No.242388F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84884-5-PBS targets MTHFD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTHFD1 fusion protein Ag1225 相同性解析による交差性が予測される生物種
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 101-110 kDa
GenBank accession numberBC009806
Gene Symbol MTHFD1
Gene ID (NCBI) 4522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP11586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)