Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16113-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 16113-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 16113-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16113-1-AP
IP result of anti-MTHFD1L (IP:16113-1-AP, 4ug; Detection:16113-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of human liver cancer using 16113-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16113-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16113-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16113-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16113-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16113-1-AP (MTHFD1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

MTHFD1L Polyclonal antibody

MTHFD1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16113-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, COLO 320 cells, mouse ovary tissue, HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 18 publications below
IHC	See 2 publications below

Product Information

16113-1-AP targets MTHFD1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MTHFD1L fusion protein Ag9036 相同性解析による交差性が予測される生物種
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Calculated molecular weight	978 aa, 106 kDa
Observed molecular weight	106 kDa
GenBank accession number	BC017477
Gene Symbol	MTHFD1L
Gene ID (NCBI)	25902
RRID	AB_2250974
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6UB35
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MTHFD1L antibody 16113-1-AP	Download protocol
IHC protocol for MTHFD1L antibody 16113-1-AP	Download protocol
IF protocol for MTHFD1L antibody 16113-1-AP	Download protocol
IP protocol for MTHFD1L antibody 16113-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions. Authors - Saara Forsström View Article
human	WB	Cell Metab Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability. Authors - Won Dong Lee View Article
human	WB	Nat Metab Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells Authors - Alanna C Green View Article
human, mouse	WB	Cell Metab Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. Authors - Joni Nikkanen View Article
human	WB, IHC	J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. Authors - Li Cui KD Validated View Article
human	WB	Nat Commun Increased formate overflow is a hallmark of oxidative cancer. Authors - Johannes Meiser View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16113-1-AP

WB analysis of COLO 320 using 16113-1-AP

WB analysis of mouse ovary using 16113-1-AP

IP experiment of HeLa using 16113-1-AP

IHC staining of human liver cancer using 16113-1-AP

IHC staining of human liver cancer using 16113-1-AP

IHC staining of human liver cancer using 16113-1-AP

IHC staining of human liver cancer using 16113-1-AP

IF Staining of HepG2 using 16113-1-AP

MTHFD1L Polyclonal antibody

MTHFD1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.

Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Increased formate overflow is a hallmark of oxidative cancer.