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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTHFD1L Monoclonal antibody, PBS Only (Capture)

MTHFD1L Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1F2

Cat no : 68321-1-PBS

Synonyms

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3, 1G1F2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFD1L Monoclonal antibody (68321-1-Ig)

    WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTHFD1L Monoclonal antibody (68321-1-Ig)

    WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MTHFD1L Monoclonal antibody (68321-1-Ig)

    IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MTHFD1L Monoclonal antibody (68321-1-Ig)

    IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using MTHFD1L Monoclonal antibody (68321-1-Ig)

    FC experiment of HepG2 using 68321-1-Ig (same clone as 68321-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MTHFD1L Monoclonal antibody (68321-1-Ig, Clone:1G1F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50271-2

    Cytometric bead array standard curve of MP50271-2

    Cytometric bead array standard curve of MP50271-2, MTHFD1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68321-1-PBS. Detection antibody: 68321-3-PBS. Standard:Ag9061. Range: 0.195-50 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68321-1-PBS targets MTHFD1L as part of a matched antibody pair:

MP50271-2: 68321-1-PBS capture and 68321-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MTHFD1L fusion protein Ag9061 相同性解析による交差性が予測される生物種
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Calculated molecular weight 978 aa, 106 kDa
Observed molecular weight106 kDa
GenBank accession numberBC017477
Gene symbol MTHFD1L
Gene ID (NCBI) 25902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.