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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using MTHFR Monoclonal antibody (66612-1-Ig)

    WB analysis of LNCaP using 66612-1-Ig (same clone as 66612-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MTHFR Monoclonal antibody (66612-1-Ig)

    WB analysis of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MTHFR Monoclonal antibody (66612-1-Ig)

    WB analysis of HepG2 using 66612-1-Ig (same clone as 66612-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MTHFR Monoclonal antibody (66612-1-Ig)

    WB analysis of Jurkat using 66612-1-Ig (same clone as 66612-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MTHFR Monoclonal antibody (66612-1-Ig)

    IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MTHFR Monoclonal antibody (66612-1-Ig)

    IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTHFR Monoclonal antibody (66612-1-Ig)

    IF Staining of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTHFR antibody (66612-1-Ig, Clone: 1F7A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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MTHFR Monoclonal antibody, PBS Only

MTHFR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66612-1-PBS
Clone No.1F7A3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66612-1-PBS targets MTHFR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MTHFR fusion protein Ag14970 相同性解析による交差性が予測される生物種
Full Name 5,10-methylenetetrahydrofolate reductase (NADPH)
Calculated molecular weight 656 aa, 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053509
Gene Symbol MTHFR
Gene ID (NCBI) 4524
RRIDAB_2881972
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42898
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)