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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTHFR Recombinant antibody, PBS Only

MTHFR Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240348C12

Cat no : 83372-7-PBS

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348C12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFR Recombinant antibody (83372-7-RR)

    WB analysis using 83372-7-RR (same clone as 83372-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83372-7-RR (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83372-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MTHFR Recombinant antibody (83372-7-RR)

    FC experiment of HeLa using 83372-7-RR (same clone as 83372-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTHFR Recombinant antibody (83372-7-RR, Clone:240348C12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83372-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83372-7-RR

    Affinity and Kinetic Characterization of 83372-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83372-7-RR against Human MTHFR were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83372-7-PBS is MTHFR Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTHFR fusion protein Ag14970 相同性解析による交差性が予測される生物種
Full Name 5,10-methylenetetrahydrofolate reductase (NADPH)
Calculated molecular weight 656 aa, 75 kDa
GenBank accession numberBC053509
Gene symbol MTHFR
Gene ID (NCBI) 4524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.