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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTHFR Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.78-100 ng/mL

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00372-1

Print datasheet

Synonyms

MTHFR

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00372-1, Human MTHFR Recombinant Matched Antibody Pair - PBS only. 83372-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14970. 83372-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

    Sandwich ELISA standard curve of MP00372-1

    Sandwich ELISA standard curve of MP00372-1, Human MTHFR Recombinant Matched Antibody Pair - PBS only. 83372-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14970. 83372-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • K-562, Hela and HepG2 cell lysates were measured. The human MTHFR concentration of detected samples was determined to be 88.86 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate, 57.89 ng/mL (based on a 3.2 mg/mL extract load) in Hela cell lysate and 55.55 ng/mL (based on a 5 mg/mL extract load) in HepG2 cell lysate.

    Sample test of MP00372-1

    K-562, Hela and HepG2 cell lysates were measured. The human MTHFR concentration of detected samples was determined to be 88.86 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate, 57.89 ng/mL (based on a 3.2 mg/mL extract load) in Hela cell lysate and 55.55 ng/mL (based on a 5 mg/mL extract load) in HepG2 cell lysate.

  • Cytometric bead array standard curve of MP00372-1, MTHFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83372-5-PBS. Detection antibody: 83372-4-PBS. Standard: Ag14970. Range: 0.78-100 ng/mL.

    Cytometric bead array standard curve of MP00372-1

    Cytometric bead array standard curve of MP00372-1, MTHFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83372-5-PBS. Detection antibody: 83372-4-PBS. Standard: Ag14970. Range: 0.78-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00372-1 targets MTHFR in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.78-100 ng/mL (Cytometric Bead Array); 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: MTHFR Recombinant antibody, PBS Only (Capture) 83372-5-PBS (240348C3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: MTHFR Recombinant antibody, PBS Only (Detector) 83372-4-PBS (240348H7). 100 μg. Concentration 1 mgl/ml.

Alternative MTHFR matched antibody pairs: MP00372-2, MP00372-3

Matched pair protein standard: Ag14970

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83372-5-PBS
Tested Reactivity human
Immunogen MTHFR fusion protein Ag14970
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5,10-methylenetetrahydrofolate reductase (NADPH)
Synonyms Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348C3
GenBank accession numberBC053509
Gene symbol MTHFR
UniprotP42898
Gene ID (NCBI) 4524
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83372-4-PBS
Tested Reactivity human
Immunogen MTHFR fusion protein Ag14970
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5,10-methylenetetrahydrofolate reductase (NADPH)
Synonyms Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 240348H7
GenBank accession numberBC053509
Gene symbol MTHFR
UniprotP42898
Gene ID (NCBI) 4524
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.