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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis using 17552-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of HeLa using 17552-1-AP

    WB result of MTMR12 antibody (17552-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and MTMR12 knockout HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of HEK-293 using 17552-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of K-562 using 17552-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of HEK-293 using 17552-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of mouse brain using 17552-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis using 17552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of mouse lung using 17552-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MTMR12 Polyclonal antibody (17552-1-AP)

    WB analysis of K-562 using 17552-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using MTMR12 Polyclonal antibody (17552-1-AP)

    IP experiment of K-562 using 17552-1-AP

    IP result of anti-MTMR12 (IP:17552-1-AP, 4ug; Detection:17552-1-AP 1:500) with K-562 cells lysate 2400 ug.

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  • KD/KO Validated

MTMR12 Polyclonal antibody

MTMR12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17552-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

KIAA1682, Inactive phosphatidylinositol 3-phosphatase 12, 3-phosphatase adapter protein, 3-PAP, 3 phosphatase adapter protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue, mouse lung tissue, HeLa cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17552-1-AP targets MTMR12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR12 fusion protein Ag11710 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 12
Calculated molecular weight 86 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC057393
Gene Symbol MTMR12
Gene ID (NCBI) 54545
RRIDAB_2147824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0I1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MTMR12 antibody 17552-1-APDownload protocol
IP protocol for MTMR12 antibody 17552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

FASEB Bioadv

In search of alternatively spliced alpha-Klotho Kl1 protein in mouse brain

Authors - Liping Li
View Article