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  • Featured Product
  • KD/KO Validated

MTMR14 Polyclonal antibody

MTMR14 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14973-1-AP

Synonyms

Myotubularin-related protein 14, hJumpy, hEDTP, HCV NS5A-transactivated protein 4 splice variant A-binding protein 1, EC:3.1.3.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTMR14 Polyclonal antibody (14973-1-AP)

    WB analysis using 14973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTMR14 Polyclonal antibody (14973-1-AP)

    IF Staining of HeLa using 14973-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR14 antibody (14973-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14973-1-AP targets MTMR14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR14 fusion protein Ag6883 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 14
Calculated molecular weight 72 kDa
Observed molecular weight60-72 kDa
GenBank accession numberBC050626
Gene symbol MTMR14
Gene ID (NCBI) 64419
RRIDAB_2147828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa.

Protocols

Product Specific Protocols
WB protocol for MTMR14 antibody 14973-1-APDownload protocol
IF protocol for MTMR14 antibody 14973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

Knockdown MTMR14 promotes cell apoptosis and inhibits migration in liver cancer cells.

Authors - Zhaodong Li
  • KD Validated
View Article