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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTMR14 Monoclonal antibody, PBS Only

MTMR14 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8B2

Cat no : 67561-1-PBS

Synonyms

C3orf29, FLJ22405, FLJ46453, FLJ90311, hEDTP, HJUMPY, MTMR14, NS5ATP4ABP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MTMR14 Monoclonal antibody (67561-1-Ig)

    WB analysis using 67561-1-Ig (same clone as 67561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67561-1-Ig (MTMR14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MTMR14 Monoclonal antibody (67561-1-Ig)

    IHC staining of mouse heart using 67561-1-Ig (same clone as 67561-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MTMR14 Monoclonal antibody (67561-1-Ig)

    IHC staining of mouse skeletal muscle using 67561-1-Ig (same clone as 67561-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67561-1-Ig (MTMR14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using MTMR14 Monoclonal antibody (67561-1-Ig)

    IF Staining of mouse heart using 67561-1-Ig (same clone as 67561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MTMR14 antibody (67561-1-Ig, Clone: 1A8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67561-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67561-1-PBS targets MTMR14 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MTMR14 fusion protein Ag6883 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 14
Calculated molecular weight 72 kDa
Observed molecular weight74 kDa
GenBank accession numberBC050626
Gene symbol MTMR14
Gene ID (NCBI) 64419
RRIDAB_2882775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa, and also can be detected as a higher molecular weight due to the heterodimerization with other MTMR members.