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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTMR9 Recombinant antibody (83694-4-RR)

    WB analysis using 83694-4-RR (same clone as 83694-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTMR9 Recombinant antibody (83694-4-RR)

    IF Staining of HeLa using 83694-4-RR (same clone as 83694-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using MTMR9 Recombinant antibody (83694-4-RR)

    FC experiment of A549 using 83694-4-RR (same clone as 83694-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MTMR9 Recombinant antibody (83694-4-RR)

    FC experiment of HeLa using 83694-4-RR (same clone as 83694-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83694-4-RR

    Affinity and Kinetic Characterization of 83694-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

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MTMR9 Recombinant antibody, PBS Only

MTMR9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83694-4-PBS
Clone No.240167G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

myotubularin related protein 9, MTMR8, LIP STYX, Inactive phosphatidylinositol 3-phosphatase 9, C8orf9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83694-4-PBS targets MTMR9 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTMR9 fusion protein Ag34134 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 9
Calculated molecular weight 63 kDa
Observed molecular weight~60 kDa
GenBank accession numberBC022003
Gene Symbol MTMR9
Gene ID (NCBI) 66036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96QG7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).