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  • Featured Product
  • KD/KO Validated

mTOR Polyclonal antibody

mTOR Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20657-1-AP

Synonyms

FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using mTOR Polyclonal antibody (20657-1-AP)

    WB analysis of MCF-7 using 20657-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using mTOR Polyclonal antibody (20657-1-AP)

    WB analysis of MDA-MB-453s using 20657-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using mTOR Polyclonal antibody (20657-1-AP)

    WB analysis of HeLa using 20657-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using mTOR Polyclonal antibody (20657-1-AP)

    IHC staining of mouse testis using 20657-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using mTOR Polyclonal antibody (20657-1-AP)

    IHC staining of mouse testis using 20657-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using mTOR Polyclonal antibody (20657-1-AP)

    IHC staining of human breast cancer using 20657-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using mTOR Polyclonal antibody (20657-1-AP)

    IHC staining of human breast cancer using 20657-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using mTOR Polyclonal antibody (20657-1-AP)

    IF Staining of HeLa using 20657-1-AP

    Immunofluorescent analysis of HeLa cells using 20657-1-AP (MTOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inmouse testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20657-1-AP targets mTOR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, bovine, hamster, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight
GenBank accession numberNM_004958
Gene symbol mTOR
Gene ID (NCBI) 2475
RRIDAB_10734440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359). The antibody is specific to MTOR.

Protocols

Product Specific Protocols
IHC protocol for mTOR antibody 20657-1-APDownload protocol
IF protocol for mTOR antibody 20657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
View Article
mouseWB

J Exp Med

Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.

Authors - Zhe Chen
View Article
humanWB

Cell Death Differ

PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma.

Authors - Xu Zhao
View Article
humanWB

Autophagy

FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma.

Authors - Tianyu Han
View Article
ratIF

Autophagy

Autophagy and post-ischemic conditioning in retinal ischemia.

Authors - Biji Mathew
View Article
humanWB

Int J Biol Macromol

Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient mice

Authors - Han Zhao
View Article