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mTOR Polyclonal antibody

Name: mTOR Polyclonal antibody
Brand: Proteintech
SKU: 28273-1-AP
Rating: 5.00 (1 reviews)

mTOR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (7)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

106

Reviews (1)

Cat no : 28273-1-AP

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 28273-1-AP
WB result of mTOR antibody (28273-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells.
WB analysis using 28273-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28273-1-AP (mTOR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 28273-1-AP
Non-treated and Rapamycin treated lysates were subjected to SDS PAGE followed by western blot with 28881-1-AP (Phospho-mTOR (Ser2448) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (28273-1-AP) and Vinculin (66305-1-Ig) subsequently.
WB analysis of PC-3 using 28273-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 28273-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 28273-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 28273-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 28273-1-AP (MTOR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 28273-1-AP
IP result of anti-mTOR (IP:28273-1-AP, 4ug; Detection:28273-1-AP 1:5000) with HeLa cells lysate 1720 ug.
IHC staining of human prostate cancer using 28273-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 28273-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 28273-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 28273-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28273-1-AP (MTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 28273-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (28273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 28273-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (28273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 28273-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Rapamycin treated HEK-293T cells, PC-3 cells, MCF-7 cells, Rapamycin treated MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 104 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

28273-1-AP targets mTOR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human, mouse, rat, rabbit, monkey, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	mTOR fusion protein Ag28395 相同性解析による交差性が予測される生物種
Full Name	FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight	289 kDa
Observed molecular weight	250-289 kDa
GenBank accession number	NM_004958
Gene symbol	mTOR
Gene ID (NCBI)	2475
RRID	AB_2881102
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

Protocols

Product Specific Protocols
WB protocol for mTOR antibody 28273-1-AP	Download protocol
IHC protocol for mTOR antibody 28273-1-AP	Download protocol
IF protocol for mTOR antibody 28273-1-AP	Download protocol
IP protocol for mTOR antibody 28273-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Brain Behav Immun l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation. Authors - Danqing Xin View Article
mouse	WB	Proc Natl Acad Sci U S A Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagy Authors - Wenjin Yan View Article
human	WB	Phytomedicine Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells Authors - Maolei Xu View Article
human	WB	Free Radic Biol Med β-Sitosterol targets ASS1 for Nrf2 ubiquitin-dependent degradation, inducing ROS-mediated apoptosis via the PTEN/PI3K/AKT signaling pathway in ovarian cancer Authors - Haoyu Wang View Article
human	WB	J Transl Med Fasting mimicking diet inhibits tumor-associated macrophage survival and pro-tumor function in hypoxia: implications for combination therapy with anti-angiogenic agent Authors - Lei Wang View Article
human	WB	Inflamm Res Mammalian STE20-like kinase 1 inhibits synoviocytes activation in rheumatoid arthritis through mitochondrial dysfunction mediated by SIRT3/mTOR axis Authors - Min Tan View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, incubated with AF594 at RT for 2h.

Applications: Immunofluorescence
Primary Antibody Dilution: 1:200
Cell Tissue Type: Ovarian tissue

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

mTOR Polyclonal antibody

mTOR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 28273-1-AP

WB analysis using 28273-1-AP

WB analysis using 28273-1-AP

WB analysis of PC-3 using 28273-1-AP

WB analysis of MCF-7 using 28273-1-AP

WB analysis of HepG2 using 28273-1-AP

WB analysis using 28273-1-AP

IP experiment of HeLa using 28273-1-AP

IHC staining of human prostate cancer using 28273-1-AP

IHC staining of human prostate cancer using 28273-1-AP

IHC staining of human breast cancer using 28273-1-AP

IHC staining of human breast cancer using 28273-1-AP

IF Staining of HeLa using 28273-1-AP

IF Staining of HepG2 using 28273-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.

Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagy

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells

β-Sitosterol targets ASS1 for Nrf2 ubiquitin-dependent degradation, inducing ROS-mediated apoptosis via the PTEN/PI3K/AKT signaling pathway in ovarian cancer

Fasting mimicking diet inhibits tumor-associated macrophage survival and pro-tumor function in hypoxia: implications for combination therapy with anti-angiogenic agent

Mammalian STE20-like kinase 1 inhibits synoviocytes activation in rheumatoid arthritis through mitochondrial dysfunction mediated by SIRT3/mTOR axis

Reviews