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  • Featured Product
  • KD/KO Validated

MTSS1 Polyclonal antibody

MTSS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13995-1-AP

Synonyms

MIMA, MIM, Metastasis suppressor YGL-1, Metastasis suppressor YGL 1, metastasis suppressor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTSS1 Polyclonal antibody (13995-1-AP)

    WB analysis using 13995-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13995-1-AP (MTSS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MTSS1 Polyclonal antibody (13995-1-AP)

    IHC staining of human lung cancer using 13995-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MTSS1 Polyclonal antibody (13995-1-AP)

    IHC staining of human lung cancer using 13995-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTSS1 Polyclonal antibody (13995-1-AP)

    IHC staining of human liver cancer using 13995-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTSS1 Polyclonal antibody (13995-1-AP)

    IHC staining of human liver cancer using 13995-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MTSS1 Polyclonal antibody (13995-1-AP)

    IF Staining of MCF-7 using 13995-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1 antibody (13995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13995-1-AP targets MTSS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTSS1 fusion protein Ag5080 相同性解析による交差性が予測される生物種
Full Name metastasis suppressor 1
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC023998
Gene symbol MTSS1
Gene ID (NCBI) 9788
RRIDAB_3085428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTSS1 (metastasis suppressor 1), also known as MIM (missing-in-metastasis), MIM-B, BEG4 (Basal cell carcinoma-enriched gene 4) or KIAA0429, is one of the defining members of the I-BAR family of negative membrane curvature-sensing proteins first identified as being deleted in metastatic bladder cancer. MTSS1 over-expression is associated with enhanced cell migration, resulting in tumorigenesis, invasion and metastasis (PMID: 27230279). It is reported that the expression of MTSS1 is associated with poor prognosis in various cancers,such as bladder, gastric, breast and so on (PMID: 32052679).

Protocols

Product Specific Protocols
WB protocol for MTSS1 antibody 13995-1-APDownload protocol
IHC protocol for MTSS1 antibody 13995-1-APDownload protocol
IF protocol for MTSS1 antibody 13995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cytoskeleton (Hoboken)

Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.

Authors - Lijun Liang
  • KD Validated
View Article
ratIF

ACS Nano

Nonswellable Hydrogel Patch with Tissue-Mimetic Mechanical Characteristics Remodeling In Vivo Microenvironment for Effective Adhesion Prevention

Authors - Xuezhe Liu
View Article