MUC20 Monoclonal antibody
MUC20 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human, canine
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G9B2
Cat no : 68714-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MDCK cells, HEK-293 cells, HK-2 cells, A549 cells, SK-BR-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68714-1-Ig targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MUC20 fusion protein Ag31840 相同性解析による交差性が予測される生物種 |
| Full Name | mucin 20, cell surface associated |
| Calculated molecular weight | 55 kDa, 71 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC029267 |
| Gene symbol | MUC20 |
| Gene ID (NCBI) | 200958 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MUC20 antibody 68714-1-Ig | Download protocol |
| IF protocol for MUC20 antibody 68714-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
