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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MUC4 Polyclonal antibody

MUC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28703-1-AP

Synonyms

Mucin 4, MUC-4, MUC 4, ASGP 2, ASGP 1 Mucin 4 beta chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC4 Polyclonal antibody (28703-1-AP)

    WB analysis using 28703-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28703-1-AP (MUC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human prostate cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human prostate cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human colon using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human breast cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human breast cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human colon cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human colon cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human lung cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human lung cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human pancreas cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MUC4 Polyclonal antibody (28703-1-AP)

    IHC staining of human pancreas cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28703-1-AP targets MUC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC4 fusion protein Ag29799 相同性解析による交差性が予測される生物種
Full Name mucin 4, cell surface associated
Calculated molecular weight 231 kDa
Observed molecular weight231 kDa, 113 kDa
GenBank accession numberNM_018406
Gene symbol MUC4
Gene ID (NCBI) 4585
RRIDAB_2918192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface.

Protocols

Product Specific Protocols
WB protocol for MUC4 antibody 28703-1-APDownload protocol
IHC protocol for MUC4 antibody 28703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols