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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC5AC Polyclonal antibody (30408-1-AP)

    WB analysis using 30408-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30408-1-AP (MUC5AC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MUC5AC Polyclonal antibody (30408-1-AP)

    IHC staining of human stomach using 30408-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MUC5AC Polyclonal antibody (30408-1-AP)

    IHC staining of human stomach using 30408-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MUC5AC Polyclonal antibody (30408-1-AP)

    IHC staining of human stomach using 30408-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using MUC5AC Polyclonal antibody (30408-1-AP)

    IHC staining of human stomach using 30408-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MUC5AC Polyclonal antibody

MUC5AC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30408-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MUC5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30408-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC5AC fusion protein Ag30190 相同性解析による交差性が予測される生物種
Full Name mucin 5AC, oligomeric mucus/gel-forming
Calculated molecular weight 586 kDa
Observed molecular weight290 kDa
GenBank accession numberNM_001304359
Gene Symbol MUC5AC
Gene ID (NCBI) 4586
RRIDAB_3669718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98088
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC5AC (Mucin-5AC ) is a gel-forming glycoprotein of the gastric and respiratory epithelium. It can protect mucous membranes from infection and chemical damage by binding to inhaled microorganisms and particles, which are then cleared by the mucociliary system. (PubMed:14535999,PubMed:14718370)

Protocols

Product Specific Protocols
WB protocol for MUC5AC antibody 30408-1-APDownload protocol
IHC protocol for MUC5AC antibody 30408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols