MUM1 Polyclonal antibody
MUM1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 12682-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, HT-1080 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12682-1-AP targets MUM1 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MUM1 fusion protein Ag3371 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma associated antigen (mutated) 1 |
| Calculated molecular weight | 18 kDa, 79 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC008098 |
| Gene symbol | MUM1 |
| Gene ID (NCBI) | 84939 |
| RRID | AB_10642694 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MUM1 antibody 12682-1-AP | Download protocol |
| IP protocol for MUM1 antibody 12682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Aurelie (Verified Customer) (09-02-2019) | Lysats (20µg) from Mouse cortex were loaded in a 8% SDS-PAGE and immunoblotted using anti-MUM1 antibody ( 1/1000) over-night at 4°C.
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