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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MUTYH Polyclonal antibody (15237-1-AP)

    WB analysis of K-562 using 15237-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using MUTYH Polyclonal antibody (15237-1-AP)

    WB analysis of Raji using 15237-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MUTYH Polyclonal antibody (15237-1-AP)

    WB analysis of HeLa using 15237-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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MUTYH Polyclonal antibody

MUTYH Polyclonal Antibody for WB, ELISA
Cat No. 15237-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15237-1-AP targets MUTYH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUTYH fusion protein Ag7359 相同性解析による交差性が予測される生物種
Full Name mutY homolog (E. coli)
Calculated molecular weight 60 kDa
Observed molecular weight 53 kDa, 65 kDa
GenBank accession numberBC003178
Gene Symbol MUTYH
Gene ID (NCBI) 4595
RRIDAB_2147408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Protocols

Product Specific Protocols
WB protocol for MUTYH antibody 15237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols