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  • Featured Product
  • KD/KO Validated

MUTYH Polyclonal antibody

MUTYH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19650-1-AP

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MUTYH Polyclonal antibody (19650-1-AP)

    WB analysis of HeLa using 19650-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MUTYH Polyclonal antibody (19650-1-AP)

    WB analysis of Jurkat using 19650-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using MUTYH Polyclonal antibody (19650-1-AP)

    WB analysis of mouse thymus using 19650-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MUTYH Polyclonal antibody (19650-1-AP)

    WB analysis of K-562 using 19650-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using MUTYH Polyclonal antibody (19650-1-AP)

    WB analysis of Raji using 19650-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using MUTYH Polyclonal antibody (19650-1-AP)

    WB analysis of COLO 320 using 19650-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MUTYH Polyclonal antibody (19650-1-AP)

    IP experiment of HeLa using 19650-1-AP

    IP result of anti-MUTYH (IP:19650-1-AP, 4ug; Detection:19650-1-AP 1:500) with HeLa cells lysate 3440ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MUTYH Polyclonal antibody (19650-1-AP)

    IHC staining of human kidney using 19650-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MUTYH Polyclonal antibody (19650-1-AP)

    IHC staining of mouse liver using 19650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MUTYH Polyclonal antibody (19650-1-AP)

    IHC staining of mouse liver using 19650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MUTYH Polyclonal antibody (19650-1-AP)

    IHC staining of human kidney using 19650-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUTYH Polyclonal antibody (19650-1-AP)

    IHC staining of human colon cancer using 19650-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MUTYH Polyclonal antibody (19650-1-AP)

    IHC staining of human colon cancer using 19650-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, Jurkat cells, K-562 cells, mouse thymus tissue, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, human kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19650-1-AP targets MUTYH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUTYH fusion protein Ag7526 相同性解析による交差性が予測される生物種
Full Name mutY homolog (E. coli)
Calculated molecular weight 60 kDa
Observed molecular weight 53-64 kDa
GenBank accession numberBC003178
Gene symbol MUTYH
Gene ID (NCBI) 4595
RRIDAB_10694143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Protocols

Product Specific Protocols
WB protocol for MUTYH antibody 19650-1-APDownload protocol
IHC protocol for MUTYH antibody 19650-1-APDownload protocol
IP protocol for MUTYH antibody 19650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease

Authors - Yuri Mizuno
View Article
humanWB

Oncol Rep

Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.

Authors - Yanxia Guo
  • KD Validated
View Article
humanWB

Cell Death Dis

Potent and specific MTH1 inhibitors targeting gastric cancer.

Authors - Wenjuan Zhou
View Article