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  • Featured Product
  • KD/KO Validated

MX1 Polyclonal antibody

MX1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 13750-1-AP

Synonyms

MX 1, Interferon-regulated resistance GTP-binding protein MxA, Interferon-induced protein p78, Interferon-induced GTP-binding protein Mx1, N-terminally processed, Interferon-induced GTP-binding protein Mx1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MX1 Polyclonal antibody (13750-1-AP)

    WB analysis using 13750-1-AP

    Lysates of A549 cells treated or non-treated with IFN-alpha were subjected to SDS PAGE followed by western blot with 13750-1-AP (MX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MX1 Polyclonal antibody (13750-1-AP)

    WB analysis of mouse lung using 13750-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13750-1-AP (MX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using MX1 Polyclonal antibody (13750-1-AP)

    IP experiment of A549 cells using 13750-1-AP

    IP result of anti-MX1 (13750-1-AP for IP and Detection) with A549 cell.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MX1 Polyclonal antibody (13750-1-AP)

    IHC staining of human tonsillitis using 13750-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13750-1-AP (MX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MX1 Polyclonal antibody (13750-1-AP)

    IHC staining of human lung cancer using 13750-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 13750-1-AP (MX1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MX1 Polyclonal antibody (13750-1-AP)

    IHC staining of human lung cancer using 13750-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 13750-1-AP (MX1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MX1 Polyclonal antibody (13750-1-AP)

    IF Staining of A549 using 13750-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (13750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A549 cells using MX1 Polyclonal antibody (13750-1-AP)

    FC experiment of A549 using 13750-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.4 ug MX1 Polyclonal antibody (13750-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, mouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13750-1-AP targets MX1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig, canine, monkey, chicken, hamster, pavo cristatus, macaque
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MX1 fusion protein Ag4692 相同性解析による交差性が予測される生物種
Full Name Myxovirus resistance protein 1
Calculated molecular weight 662 aa, 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC032602
Gene symbol MX1
Gene ID (NCBI) 4599
RRIDAB_2266768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.

Protocols

Product Specific Protocols
WB protocol for MX1 antibody 13750-1-APDownload protocol
IHC protocol for MX1 antibody 13750-1-APDownload protocol
IF protocol for MX1 antibody 13750-1-APDownload protocol
FC protocol for MX1 antibody 13750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

IFITM3 restricts the morbidity and mortality associated with influenza.

Authors - Everitt AR
View Article
humanWB

Cell Host Microbe

NRAV, a long noncoding RNA, modulates antiviral responses through suppression of interferon-stimulated gene transcription.

Authors - Jing Ouyang
View Article
human

Nat Commun

Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.

Authors - Wei Liu
View Article
humanWB

Hepatology

MxA inhibits hepatitis B virus replication by interaction with hepatitis B core antigen.

Authors - Li Ning N
View Article
macaqueIHC

Nat Commun

Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques.

Authors - Shaheed A Abdulhaqq
View Article
humanWB

J Hepatol

PMID: 25481566

Authors - Shi Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hannah (Verified Customer) (09-21-2020)

Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
MX1 Antibody Western Blot validation (1/500 dilution) in (Cat no:13750-1-AP)