Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
Product Information
25472-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | matrix-remodelling associated 5 |
| Calculated molecular weight | 312 kDa |
| Observed molecular weight | 163 kDa, 312 kDa |
| GenBank accession number | NM_015419 |
| Gene Symbol | MXRA5 |
| Gene ID (NCBI) | 25878 |
| RRID | AB_2918088 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR99 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MXRA5 antibody 25472-1-AP | Download protocol |
| IHC protocol for MXRA5 antibody 25472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells
| ||
Am J Cancer Res Single-cell analysis reveals one cancer-associated fibroblasts subtype linked to metastasis in breast cancer: MXRA5 as a potential novel marker for prognosis | ||
J Cancer Identification of an Immune signature assisted prognosis, and immunotherapy prediction for IDH wildtype glioblastoma |