• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

MXRA5 Polyclonal antibody

MXRA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25472-1-AP

Synonyms

Adlican, MXRA5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MXRA5 Polyclonal antibody (25472-1-AP)

    WB analysis using 25472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25472-1-AP (MXRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human kidney using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human colon cancer using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of mouse liver using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of mouse liver using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human colon cancer using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human kidney using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human liver using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MXRA5 Polyclonal antibody (25472-1-AP)

    IHC staining of human liver using 25472-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25472-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name matrix-remodelling associated 5
Calculated molecular weight 312 kDa
Observed molecular weight163 kDa, 312 kDa
GenBank accession numberNM_015419
Gene symbol MXRA5
Gene ID (NCBI) 25878
RRIDAB_2918088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751).

Protocols

Product Specific Protocols
WB protocol for MXRA5 antibody 25472-1-APDownload protocol
IHC protocol for MXRA5 antibody 25472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Sci Rep

Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells

Authors - Kazuma Yoshida
  • KD Validated
View Article
humanIHC

Am J Cancer Res

Single-cell analysis reveals one cancer-associated fibroblasts subtype linked to metastasis in breast cancer: MXRA5 as a potential novel marker for prognosis

Authors - Huifang Deng
View Article