Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells, MOLT-4 cells |
| Positive IP detected in | HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
17800-1-AP targets MYB/c-Myb in WB, IP, IF, RIP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYB/c-Myb fusion protein Ag12021 相同性解析による交差性が予測される生物種 |
| Full Name | v-myb myeloblastosis viral oncogene homolog (avian) |
| Calculated molecular weight | 640 aa, 72 kDa |
| Observed molecular weight | 40-50 kDa, 72-80 kDa |
| GenBank accession number | BC064955 |
| Gene Symbol | c-Myb |
| Gene ID (NCBI) | 4602 |
| RRID | AB_2148029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10242 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYB/c-Myb antibody 17800-1-AP | Download protocol |
| IP protocol for MYB/c-Myb antibody 17800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly. | ||
Nat Commun Tcf1 and Lef1 provide constant supervision to mature CD8+ T cell identity and function by organizing genomic architecture. | ||
RNA Biol SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma. | ||
Front Endocrinol (Lausanne) KRT17 from skin cells with high glucose stimulation promotes keratinocytes proliferation and migration | ||
Front Med (Lausanne) Lidocaine and Bupivacaine Downregulate MYB and DANCR lncRNA by Upregulating miR-187-5p in MCF-7 Cells. | ||
Cancer Genomics Proteomics Myb Repression Mediates Stat5b-knockdown-induced Apoptosis and Inhibits Proliferation of Glioblastoma Stem Cells |