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c-MYC Polyclonal antibody

Name: c-MYC Polyclonal antibody
Brand: Proteintech
SKU: 10828-1-AP
Rating: 4.00 (4 reviews)

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, EMSA

Conjugate

Unconjugated

Publications

670

Reviews (4)

Cat no : 10828-1-AP

Synonyms

MYC, Myc proto oncogene protein, MRTL, Class E basic helix-loop-helix protein 39, c Myc

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of A549 using 10828-1-AP
WB result of c-MYC antibody (10828-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-MYC transfected A549 cells.
WB analysis using 10828-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10828-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10828-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10828-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10828-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10828-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10828-1-AP
IP result of anti-c-MYC (IP:10828-1-AP, 4ug; Detection:10828-1-AP 1:1000) with MCF-7 cells lysate 1660 ug.
IHC staining of human ovary cancer using 10828-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 10828-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using c-MYC antibody (10828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of Saos-2 using 10828-1-AP
Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of Saos-2 using 10828-1-AP
Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HEK-293 using 10828-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 10828-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (10828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 10828-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells.
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Saos-2 cells, HEK-293 cells, U2OS cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 25 publications below
WB	See 609 publications below
IF	See 39 publications below
IP	See 23 publications below
CoIP	See 15 publications below
ChIP	See 14 publications below

Product Information

10828-1-AP targets c-MYC in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, EMSA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig, chicken, sheep, p. lilacinum
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	c-MYC fusion protein Ag1263 相同性解析による交差性が予測される生物種
Full Name	v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight	49 kDa
Observed molecular weight	62-65 kDa, 50 kDa
GenBank accession number	BC000141
Gene symbol	MYC
Gene ID (NCBI)	4609
RRID	AB_2148585
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.

Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 10828-1-AP	Download protocol
IHC protocol for c-MYC antibody 10828-1-AP	Download protocol
IF protocol for c-MYC antibody 10828-1-AP	Download protocol
IP protocol for c-MYC antibody 10828-1-AP	Download protocol
FC protocol for c-MYC antibody 10828-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature RIC-seq for global in situ profiling of RNA-RNA spatial interactions. Authors - Zhaokui Cai View Article
mouse	WB	Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration Authors - Jinpeng Du KD Validated View Article
human	WB	Signal Transduct Target Ther Overexpression of CIP2A is associated with poor prognosis in multiple myeloma. Authors - Xuewen Liu View Article
human	WB,IF	Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis Authors - Xiao-Mei Yang View Article
human	WB	Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. Authors - Alessandro Carugo KD Validated View Article
	WB	Cell Metab Efferocytosis induces macrophage proliferation to help resolve tissue injury. Authors - Brennan D Gerlach KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Molly (Verified Customer) (05-02-2023)	The antibody worked well for western blotting following the recommended protocol. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Ewing sarcoma cell lines
FH Anna-Maria (Verified Customer) (08-13-2019)	This antibody worked as expected for western blotting Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Bladder cancer cell lines
FH Christine (Verified Customer) (11-13-2018)	The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions. Applications: Immunohistochemistry, Primary Antibody Dilution: 1 to 300 Cell Tissue Type: Colon
FH Claudio (Verified Customer) (12-14-2017)	We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level. Applications: Immunohistochemistry, Immunofluorescence Primary Antibody Dilution: 100 Cell Tissue Type: Mouse brain tumors with c-myc upregulation (determined at the mRNA level)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of A549 using 10828-1-AP

WB analysis using 10828-1-AP

WB analysis using 10828-1-AP

WB analysis using 10828-1-AP

WB analysis using 10828-1-AP

WB analysis of human placenta using 10828-1-AP

WB analysis of HL-60 using 10828-1-AP

IP experiment of MCF-7 using 10828-1-AP

IHC staining of human ovary cancer using 10828-1-AP

IF Staining of U2OS using 10828-1-AP

IF Staining of Saos-2 using 10828-1-AP

IF Staining of Saos-2 using 10828-1-AP

IF Staining of HEK-293 using 10828-1-AP

FC experiment of HeLa using 10828-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RIC-seq for global in situ profiling of RNA-RNA spatial interactions.

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Efferocytosis induces macrophage proliferation to help resolve tissue injury.

Reviews

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