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MYC tag Polyclonal antibody

MYC tag Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16286-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

myc, EQKLISEEDL, myc-tag

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRecombinant protein protein, Recombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16286-1-AP targets MYC tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYC tag fusion protein Ag9409 相同性解析による交差性が予測される生物種
Full Name Myc tag
Gene Symbol Myc tag
Gene ID (NCBI) 99
RRIDAB_11182162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.

What is the molecular weight of myc?

1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).

What are the applications for myc tag?

The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). 

What is the structure of myc tag?

The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.


Protocols

Product Specific Protocols
WB protocol for MYC tag antibody 16286-1-APDownload protocol
IF protocol for MYC tag antibody 16286-1-APDownload protocol
IP protocol for MYC tag antibody 16286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
WB,CoIP

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang

Nat Microbiol

Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.

Authors - Hua Zhang

Drug Resist Updat

MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance

Authors - GuoSheng Wang

Cell Stem Cell

A primate-specific endogenous retroviral envelope protein sequesters SFRP2 to regulate human cardiomyocyte development

Authors - Ran Zhang
WB,IP

Nat Commun

TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR

Authors - Xiang-Hong Ran
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