MYC tag Polyclonal antibody
MYC tag Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (4)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
376
Cat no : 16286-1-AP
Synonyms
"MYC tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | Recombinant protein protein, Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16286-1-AP targets MYC tag in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, monkey, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYC tag fusion protein Ag9409 相同性解析による交差性が予測される生物種 |
| Full Name | MYC tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11182162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IF protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IP protocol for MYC tag antibody 16286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Microbiol Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. | ||
Cell Stem Cell A primate-specific endogenous retroviral envelope protein sequesters SFRP2 to regulate human cardiomyocyte development | ||
Nat Commun TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR | ||
Nat Commun The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH RASHMI (Verified Customer) (08-26-2024) | I used this antibody recently for ABC staining, it worked great, highly recommended.
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FH RASHMI (Verified Customer) (07-26-2024) | used this product, highly recommended
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FH Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot, IF, IP
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FH Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot and IF and IP
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FH Amy (Verified Customer) (03-05-2023) | Detected overexpressed Mac-tagged proteins in HEK293T cell lysates but with a fair few background bands.
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FH James (Verified Customer) (11-30-2022) | Very good antibody
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FH Boyan (Verified Customer) (08-03-2022) | gives a good signal in IF
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FH Chahat (Verified Customer) (06-08-2022) | The myc antibody gives clear staining!
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FH WEI (Verified Customer) (03-09-2022) | Good antibody for detecting overexpression proteins in 293 cells.
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FH Minze (Verified Customer) (01-11-2022) | it works well for immunofluorecence so far.
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FH Phoebe (Verified Customer) (03-01-2019) | This antibody was very clean in a western blot to detect Myc-tagged pol2 in S cerevisiae whole cell extract and chromatin fractionation samples.
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