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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYCBP2 Polyclonal antibody

MYCBP2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 27951-1-AP

Synonyms

Protein associated with Myc, Pam/highwire/rpm 1 protein, PAM, MYC binding protein 2, KIAA0916

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of human brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of human brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of rat brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of rat brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of mouse brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MYCBP2 Polyclonal antibody (27951-1-AP)

    IHC staining of mouse brain using 27951-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27951-1-AP (MYCBP2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYCBP2 Polyclonal antibody (27951-1-AP)

    IF Staining of U2OS using 27951-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCBP2 antibody (27951-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using MYCBP2 Polyclonal antibody (27951-1-AP)

    FC experiment of U2OS using 27951-1-AP

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug MYCBP2 Polyclonal antibody (27951-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IHC detected inhuman brain tissue, rat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

27951-1-AP targets MYCBP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYCBP2 fusion protein Ag27228 相同性解析による交差性が予測される生物種
Full Name MYC binding protein 2
Calculated molecular weight 510 kDa
GenBank accession numberNM_015057
Gene symbol MYCBP2
Gene ID (NCBI) 23077
RRIDAB_2881020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MYCBP2 antibody 27951-1-APDownload protocol
IF protocol for MYCBP2 antibody 27951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

MYCBP2 expression correlated with inflammatory cell infiltration and prognosis immunotherapy in thyroid cancer patients

Authors - Guilin Wang
View Article
humanIHC

Arch Biochem Biophys

Exosomes from human umbilical cord mesenchymal stem cells protect aortas in Db/db mice characterized by combination of metabolomics and proteomics

Authors - Ming-Ying Ling
View Article