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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYCL1 Recombinant antibody (82881-1-RR)

    IF Staining of U2OS using 82881-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCL1 antibody (82881-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green).

  • Flow cytometry (FC) experiment of HeLa cells using MYCL1 Recombinant antibody (82881-1-RR)

    FC experiment of HeLa using 82881-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYCL1 Recombinant antibody (82881-1-RR, Clone:230104D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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MYCL1 Recombinant antibody

MYCL1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82881-1-RR
Clone No.230104D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Protein L-Myc, MYCL, LMYC, Class E basic helix-loop-helix protein 38, bHLHe38

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82881-1-RR targets MYCL1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYCL1 fusion protein Ag30335 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)
Calculated molecular weight 40 kDa
GenBank accession numberBC011864
Gene Symbol MYCL1
Gene ID (NCBI) 4610
RRIDAB_3670600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12524
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for MYCL1 antibody 82881-1-RRDownload protocol
FC protocol for MYCL1 antibody 82881-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols