• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYCN Recombinant antibody

MYCN Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242406D8

Cat no : 84886-3-RR

Synonyms

N-myc proto-oncogene protein, N myc proto oncogene protein, N myc, MODED, bHLHe37

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MYCN Recombinant antibody (84886-3-RR)

    WB analysis using 84886-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MYCN Recombinant antibody (84886-3-RR)

    WB analysis of human placenta using 84886-3-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using MYCN Recombinant antibody (84886-3-RR)

    FC experiment of SH-SY5Y using 84886-3-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MYCN Recombinant antibody (84886-3-RR, Clone:242406D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inIMR-32 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84886-3-RR targets MYCN in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYCN fusion protein Ag27224 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Calculated molecular weight 50 kDa
Observed molecular weight~65 kDa
GenBank accession numberBC002712
Gene symbol MYCN
Gene ID (NCBI) 4613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

Protocols

Product Specific Protocols
WB protocol for MYCN antibody 84886-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols