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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYD88 Polyclonal antibody (29946-1-AP)

    WB analysis using 29946-1-AP (same clone as 29946-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29946-1-AP (MYD88 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29946-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MYD88 Polyclonal antibody (29946-1-AP)

    IHC staining of mouse liver using 29946-1-AP (same clone as 29946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29946-1-AP (MYD88 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29946-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MYD88 Polyclonal antibody (29946-1-AP)

    IHC staining of mouse liver using 29946-1-AP (same clone as 29946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29946-1-AP (MYD88 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29946-1-PBS in a different storage buffer formulation.

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Mouse MYD88 Polyclonal antibody, PBS Only

MYD88 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29946-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29946-1-PBS targets MYD88 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYD88 fusion protein Ag30662 相同性解析による交差性が予測される生物種
Full Name myeloid differentiation primary response gene 88
Calculated molecular weight 34 kDa
Observed molecular weight29-34 kDa
GenBank accession numberNM_010851
Gene Symbol Myd88
Gene ID (NCBI) 17874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22366
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis. MYD88 is detected in bone marrow.