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MYEF2 Polyclonal antibody
MYEF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16051-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
16051-1-AP targets MYEF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYEF2 fusion protein Ag9010 相同性解析による交差性が予測される生物種 |
| Full Name | myelin expression factor 2 |
| Calculated molecular weight | 600 aa, 64 kDa |
| Observed molecular weight | 61-64 kDa |
| GenBank accession number | BC014533 |
| Gene symbol | MYEF2 |
| Gene ID (NCBI) | 50804 |
| RRID | AB_2146869 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYEF2 antibody 16051-1-AP | Download protocol |
| IHC protocol for MYEF2 antibody 16051-1-AP | Download protocol |
| IF protocol for MYEF2 antibody 16051-1-AP | Download protocol |
| IP protocol for MYEF2 antibody 16051-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination. | ||
BMC Gastroenterol Expression and function of myelin expression factor 2 in hepatocellular carcinoma
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wael (Verified Customer) (05-16-2023) | Hek293 cells were fractionated into nuclear and cytoplasmic fractions. specific band for MYEF2 between 55 and 100kd, enriched in the Nuclear fraction. Lanes order: 1-ladder 2-Nuclear fraction 3-Cytoplasmic frcation
 |
