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  • KD/KO Validated

MYEF2 Polyclonal antibody

MYEF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16051-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

MST156, MEF-2, MEF2, MEF 2, KIAA1341

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16051-1-AP targets MYEF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYEF2 fusion protein Ag9010 相同性解析による交差性が予測される生物種
Full Name myelin expression factor 2
Calculated molecular weight 600 aa, 64 kDa
Observed molecular weight61-64 kDa
GenBank accession numberBC014533
Gene Symbol MYEF2
Gene ID (NCBI) 50804
RRIDAB_2146869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2K5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYEF2 antibody 16051-1-APDownload protocol
IHC protocol for MYEF2 antibody 16051-1-APDownload protocol
IF protocol for MYEF2 antibody 16051-1-APDownload protocol
IP protocol for MYEF2 antibody 16051-1-APDownload protocol
FC protocol for MYEF2 antibody 16051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IHC, CoIP

Cell Rep

PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.

Authors - Yan Wang
humanWB,IHC

BMC Gastroenterol

Expression and function of myelin expression factor 2 in hepatocellular carcinoma

Authors - Peng Zhang
  • KD Validated
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