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  • Featured Product
  • KD/KO Validated

MYEF2 Polyclonal antibody

MYEF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16051-1-AP

Synonyms

MST156, MEF-2, MEF2, MEF 2, KIAA1341

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYEF2 Polyclonal antibody (16051-1-AP)

    WB analysis using 16051-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MYEF2 Polyclonal antibody (16051-1-AP)

    WB analysis of Jurkat using 16051-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MYEF2 Polyclonal antibody (16051-1-AP)

    WB analysis of Jurkat using 16051-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MYEF2 Polyclonal antibody (16051-1-AP)

    WB analysis of Jurkat using 16051-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MYEF2 Polyclonal antibody (16051-1-AP)

    WB analysis of Jurkat using 16051-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MYEF2 Polyclonal antibody (16051-1-AP)

    IP experiment of Jurkat using 16051-1-AP

    IP result of anti-MYEF2 (IP:16051-1-AP, 4ug; Detection:16051-1-AP 1:2000) with Jurkat cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MYEF2 Polyclonal antibody (16051-1-AP)

    IHC staining of human breast cancer using 16051-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16051-1-AP (MYEF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MYEF2 Polyclonal antibody (16051-1-AP)

    IHC staining of human breast cancer using 16051-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16051-1-AP (MYEF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYEF2 Polyclonal antibody (16051-1-AP)

    IF Staining of HepG2 using 16051-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYEF2 antibody (16051-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of C6 cells using MYEF2 Polyclonal antibody (16051-1-AP)

    FC experiment of C6 using 16051-1-AP

    1x10^6 C6 cells were intracellularly stained with 0.25 ug MYEF2 Polyclonal antibody (16051-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16051-1-AP targets MYEF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYEF2 fusion protein Ag9010 相同性解析による交差性が予測される生物種
Full Name myelin expression factor 2
Calculated molecular weight 600 aa, 64 kDa
Observed molecular weight61-64 kDa
GenBank accession numberBC014533
Gene symbol MYEF2
Gene ID (NCBI) 50804
RRIDAB_2146869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYEF2 antibody 16051-1-APDownload protocol
IHC protocol for MYEF2 antibody 16051-1-APDownload protocol
IF protocol for MYEF2 antibody 16051-1-APDownload protocol
IP protocol for MYEF2 antibody 16051-1-APDownload protocol
FC protocol for MYEF2 antibody 16051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IHC, CoIP

Cell Rep

PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.

Authors - Yan Wang
View Article
humanWB,IHC

BMC Gastroenterol

Expression and function of myelin expression factor 2 in hepatocellular carcinoma

Authors - Peng Zhang
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wael (Verified Customer) (05-16-2023)

Hek293 cells were fractionated into nuclear and cytoplasmic fractions. specific band for MYEF2 between 55 and 100kd, enriched in the Nuclear fraction. Lanes order: 1-ladder 2-Nuclear fraction 3-Cytoplasmic frcation

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
MYEF2 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:16051-1-AP)