MYF6 Polyclonal antibody
MYF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, chip, ELISA
Conjugate
Unconjugated
Cat no : 11754-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
11754-1-AP targets MYF6 in WB, IHC, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYF6 fusion protein Ag2350 相同性解析による交差性が予測される生物種 |
| Full Name | myogenic factor 6 (herculin) |
| Calculated molecular weight | 242 aa, 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC017834 |
| Gene symbol | MYF6 |
| Gene ID (NCBI) | 4618 |
| RRID | AB_2250929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYF6 antibody 11754-1-AP | Download protocol |
| IHC protocol for MYF6 antibody 11754-1-AP | Download protocol |
| IF protocol for MYF6 antibody 11754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity. | ||
Front Cell Dev Biol A Functional SNP in the Promoter of LBX1 Is Associated With the Development of Adolescent Idiopathic Scoliosis Through Involvement in the Myogenesis of Paraspinal Muscles. | ||
In Vitro Cell Dev Biol Anim Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (11-11-2023) | Worked well for frozen mouse heart tissue sections.
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