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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    WB analysis of pig skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    1 μg pig skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    WB analysis of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    1 μg mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    IHC staining of rat skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using MYH1 Monoclonal antibody (67299-1-Ig)

    IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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MYH1 Monoclonal antibody, PBS Only

MYH1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67299-1-PBS
Clone No.1G10H9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x, 1G10H9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67299-1-PBS targets MYH1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MYH1 fusion protein Ag17129 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 1, skeletal muscle, adult
Calculated molecular weight 1939 aa, 223 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC114545
Gene Symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_2882563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12882
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.