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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    WB analysis of mouse brain using 67243-1-Ig (same clone as 67243-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MYH10 Monoclonal antibody (67243-1-Ig)

    WB analysis of HEK-293 using 67243-1-Ig (same clone as 67243-1-PBS)

    WB result of MYH10 antibody (67243-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10 transfected HEK-293 cells. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    WB analysis of rat brain using 67243-1-Ig (same clone as 67243-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    WB analysis of rat cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    WB analysis of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using MYH10 Monoclonal antibody (67243-1-Ig)

    IF Staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MYH10 antibody (67243-1-Ig, Clone: 2H8G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MYH10 Monoclonal antibody, PBS Only

MYH10 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67243-1-PBS
Clone No.2H8G7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67243-1-PBS targets MYH10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MYH10 fusion protein Ag17069 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 10, non-muscle
Calculated molecular weight 1985 aa, 230 kDa
Observed molecular weight 200-230 kDa
GenBank accession numberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_2882522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35580
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.