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  • Featured Product
  • KD/KO Validated

MYH10-Specific Polyclonal antibody

MYH10-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19673-1-AP

Synonyms

Myosin 10, MYH10, NMMHC II-b, Myosin-10, Myosin heavy chain, non-muscle IIb

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MYH10-Specific Polyclonal antibody (19673-1-AP)

    WB analysis using 19673-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19673-1-AP (MYH10-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MYH10-Specific Polyclonal antibody (19673-1-AP)

    WB analysis of HEK-293 using 19673-1-AP

    WB result of MYH10-Specific antibody (19673-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10-Specific transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IP experiment of mouse brain using 19673-1-AP

    IP result of anti-MYH10-Specific (IP:19673-1-AP, 4ug; Detection:19673-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IHC staining of human cerebellum using 19673-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IHC staining of human cerebellum using 19673-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IHC staining of human brain using 19673-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IHC staining of human brain using 19673-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IF Staining of HepG2 using 19673-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 19673-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MYH10-Specific Polyclonal antibody (19673-1-AP)

    IF Staining of HepG2 using 19673-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH10-Specific antibody (19673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293T cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19673-1-AP targets MYH10-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 10, non-muscle
Calculated molecular weight 231 kDa
Observed molecular weight 229 kDa
GenBank accession numberNM_005964
Gene symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_10858231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.

Protocols

Product Specific Protocols
WB protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IHC protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IF protocol for MYH10-Specific antibody 19673-1-APDownload protocol
IP protocol for MYH10-Specific antibody 19673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan
View Article
ratWB,CoIP

Bioact Mater

Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.

Authors - Pengzhen Cheng
View Article
humanWB

J Am Heart Assoc

Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.

Authors - Jiaqi Tang
View Article
humanIHC

Clin Genet

Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.

Authors - Zhongxian Zhu
View Article
mouseWB

Sci Rep

Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.

Authors - Jeong Suk Kang
View Article
humanWB

J Dermatol Sci

Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers

Authors - Yushi Katsuyama
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ryan (Verified Customer) (02-27-2019)

Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Zebrafish Heart
MYH10-Specific Antibody Immunohistochemistry,Immunofluorescence, validation (1:200 dilution) in Zebrafish Heart (Cat no:19673-1-AP)