Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, human skeletal muscle tissue, mouse colon tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 11 publications below |
Product Information
20716-1-AP targets MYH14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy chain 14 |
| Calculated molecular weight | 228 kDa |
| Observed molecular weight | 228 kDa |
| GenBank accession number | NM_024729 |
| Gene Symbol | MYH14 |
| Gene ID (NCBI) | 79784 |
| RRID | AB_10859247 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH14 antibody 20716-1-AP | Download protocol |
| IHC protocol for MYH14 antibody 20716-1-AP | Download protocol |
| IF protocol for MYH14 antibody 20716-1-AP | Download protocol |
| IP protocol for MYH14 antibody 20716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions. | ||
J Cell Biol Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane | ||
Clin Genet Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population. | ||
Stem Cells Int Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway. | ||
Mol Biol Cell Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli. | ||
Life (Basel) Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy. |